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Amersham Life Sciences Inc 32p datp
32p Datp, supplied by Amersham Life Sciences Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/32p+datp/32p+a+dctp/us12618068-288-30-33
Average 86 stars, based on 1 article reviews
32p datp - by Bioz Stars, 2026-10
86/100 stars

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Purification:

Article Title: mRNA composition and production method for use in anti-viral and anti-cancer vaccines
Article Snippet: .. The probe is further purified by high-performance liquid chromatography (HPLC) and tail-labeled with terminal transferase (20 units) for 20 min in the presence of either a dye-labeled nucleotide analog or [32P]-dATP (>3000 Ci/mM, Amersham International, Arlington Heights, IL). ..

Article Title: Replicase cycling reaction (RCR) and the related SamRNA designs thereof
Article Snippet: .. The probe is further purified by high-performance liquid chromatography (HPLC) and tail-labeled with terminal transferase (20 units) for 20 min in the presence of either a dye-labeled nucleotide analog or [32P]-dATP (>3000 Ci/mM, Amersham International, Arlington Heights, IL). .. Protein Extraction and Western Blot Analysis Cells (106) are lysed with a CelLytic-M lysis/extraction reagent (Sigma) supplemented with protease inhibitors, Leupeptin, TLCK, TAME and PMSF, following the manufacturer's suggestion.

Article Title: Replicase cycling reaction (RCR)
Article Snippet: .. The probe is further purified by high-performance liquid chromatography (HPLC) and tail-labeled with terminal transferase (20 units) for 20 min in the presence of either a dye-labeled nucleotide analog or [32P]-dATP (>3000 Ci/mM, Amersham International, Arlington Heights, IL). .. Protein Extraction and Western Blot Analysis Cells (106) are lysed with a CelLytic-M lysis/extraction reagent (Sigma) supplemented with protease inhibitors, Leupeptin, TLCK, TAME and PMSF, following the manufacturer's suggestion.

High Performance Liquid Chromatography:

Article Title: mRNA composition and production method for use in anti-viral and anti-cancer vaccines
Article Snippet: .. The probe is further purified by high-performance liquid chromatography (HPLC) and tail-labeled with terminal transferase (20 units) for 20 min in the presence of either a dye-labeled nucleotide analog or [32P]-dATP (>3000 Ci/mM, Amersham International, Arlington Heights, IL). ..

Article Title: Replicase cycling reaction (RCR) and the related SamRNA designs thereof
Article Snippet: .. The probe is further purified by high-performance liquid chromatography (HPLC) and tail-labeled with terminal transferase (20 units) for 20 min in the presence of either a dye-labeled nucleotide analog or [32P]-dATP (>3000 Ci/mM, Amersham International, Arlington Heights, IL). .. Protein Extraction and Western Blot Analysis Cells (106) are lysed with a CelLytic-M lysis/extraction reagent (Sigma) supplemented with protease inhibitors, Leupeptin, TLCK, TAME and PMSF, following the manufacturer's suggestion.

Article Title: Replicase cycling reaction (RCR)
Article Snippet: .. The probe is further purified by high-performance liquid chromatography (HPLC) and tail-labeled with terminal transferase (20 units) for 20 min in the presence of either a dye-labeled nucleotide analog or [32P]-dATP (>3000 Ci/mM, Amersham International, Arlington Heights, IL). .. Protein Extraction and Western Blot Analysis Cells (106) are lysed with a CelLytic-M lysis/extraction reagent (Sigma) supplemented with protease inhibitors, Leupeptin, TLCK, TAME and PMSF, following the manufacturer's suggestion.

Expressing:

Article Title: Acute cadmium exposure induces stress-related gene expression in wild-type and metallothionein-I/II-null mice.
Article Snippet: This study examined the effect of acute cadmium on stress-related gene expression and free radical production in wild-type and metallothionein-I/II-null (MT-null) mice.. Atlas Toxicology arrays showed that acute cadmium (40 mol/kg as CdCl2, ip for 3 h) markedly increased the expression of genes encoding heat-shock proteins, heme oxygenase-1, and genes in response to DNA damage/repair.. The expression of genes encoding cytochrome P450 enzymes, UDP-glucuronosyltransferases, Mn-superoxide dismutase, and catalase was suppressed by cadmium.

Labeling:

Article Title: Heterocyst-Specific Expression of <i>patB</i> , a Gene Required for Nitrogen Fixation in <i>Anabaena</i> sp. Strain PCC 7120
Article Snippet: .. Probes were labeled by random priming with the Ambion Strip-Easy DNA probe synthesis kit or the Roche Random primed DNA labeling kit, using [32P]dCTP or [32P]dATP (Amersham). .. Blots were imaged by exposure to a Type BAS-III Fuji phosphorimager screen for 2 h. The exposed screen was scanned with a Molecular Dynamics Storm 860 PhosphorImager and analyzed with Molecular Dynamics IQMac software.

Stripping Membranes:

Article Title: Heterocyst-Specific Expression of <i>patB</i> , a Gene Required for Nitrogen Fixation in <i>Anabaena</i> sp. Strain PCC 7120
Article Snippet: .. Probes were labeled by random priming with the Ambion Strip-Easy DNA probe synthesis kit or the Roche Random primed DNA labeling kit, using [32P]dCTP or [32P]dATP (Amersham). .. Blots were imaged by exposure to a Type BAS-III Fuji phosphorimager screen for 2 h. The exposed screen was scanned with a Molecular Dynamics Storm 860 PhosphorImager and analyzed with Molecular Dynamics IQMac software.

Random Primed:

Article Title: Heterocyst-Specific Expression of <i>patB</i> , a Gene Required for Nitrogen Fixation in <i>Anabaena</i> sp. Strain PCC 7120
Article Snippet: .. Probes were labeled by random priming with the Ambion Strip-Easy DNA probe synthesis kit or the Roche Random primed DNA labeling kit, using [32P]dCTP or [32P]dATP (Amersham). .. Blots were imaged by exposure to a Type BAS-III Fuji phosphorimager screen for 2 h. The exposed screen was scanned with a Molecular Dynamics Storm 860 PhosphorImager and analyzed with Molecular Dynamics IQMac software.

Article Title: Immunoglobulin genes and antibody responses in the spotted wolffish (Anarhichas minor Olafsen).
Article Snippet: The spotted wol$sh Anarhichas minor Olafsen is a promising new species in aquaculture in the cold waters of northern Norway.. In this paper, some basic immunological studies of this marine species are reported.. Of comparative interest are the cDNA sequences of the immunoglobulin transcript and the antibody responses to model antigens.

DNA Labeling:

Article Title: Heterocyst-Specific Expression of <i>patB</i> , a Gene Required for Nitrogen Fixation in <i>Anabaena</i> sp. Strain PCC 7120
Article Snippet: .. Probes were labeled by random priming with the Ambion Strip-Easy DNA probe synthesis kit or the Roche Random primed DNA labeling kit, using [32P]dCTP or [32P]dATP (Amersham). .. Blots were imaged by exposure to a Type BAS-III Fuji phosphorimager screen for 2 h. The exposed screen was scanned with a Molecular Dynamics Storm 860 PhosphorImager and analyzed with Molecular Dynamics IQMac software.

Article Title: Immunoglobulin genes and antibody responses in the spotted wolffish (Anarhichas minor Olafsen).
Article Snippet: The spotted wol$sh Anarhichas minor Olafsen is a promising new species in aquaculture in the cold waters of northern Norway.. In this paper, some basic immunological studies of this marine species are reported.. Of comparative interest are the cDNA sequences of the immunoglobulin transcript and the antibody responses to model antigens.

Polymerase Chain Reaction:

Article Title: A case of Silver-Russell syndrome (SRS): multiple pituitary hormone deficiency, lack of H19 hypomethylation and favourable growth hormone (GH) treatment response.
Article Snippet: Hypomethylation of the imprinting control region 1 (ICR1) at the IGF2/H19 locus on 11p15 is linked to Silver–Russell syndrome (SRS) and/or hemihypertrophy.. This SRS patient was born in term with weight of 3500 g (50 percentile) and length 48 cm (>1 SD below the mean).. He was first noticed at the age of 10 years for short stature (114.5 cm, −3.85 SD), relatively normal head circumference, a classic facial phenotype, hemihypertrophy (2.5 cm thinner left arm and leg in comparison to the right, asymmetric face), moderate clinodactyly and striking thinness (BMI of 15.3).

other:

Article Title: IFN-alpha regulates IL-21 and IL-21R expression in human NK and T cells.
Article Snippet: Interleukin (IL)-21 is a T cell-derived cytokine that regulates innate and adaptive immune responses.. IL-21 receptor (IL-21R), which is expressed in natural killer (NK) and T cells, is structurally homologous to IL-2R and IL-15R .. These receptors also share a common cytokine receptor -chain with IL-4, IL-7, and IL-9.



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